Unraveling the Novel Bacterial Assisted Biodegradation Pathway of Morpholine

Most xenobiotics are biodegradable, persistent or recalcitrant in nature. Morpholine, a typical xenobiotic, was initially regarded as recalcitrant, however, later proved to be biodegradable by bacterial species like Mycobacterium and Pseudomonas particular. However, establishing the metabolic pathways involved for successful biodegradation of morpholine is challenging because its extreme level water solubility that affects various analytical procedures. In addition, date, no suitable methods have been reported directly estimate activity degradable products intermediates. Nevertheless, methods, especially optical density, gas chromatography mass spectrophotometric analysis, could indirectly degradation product(s) formed result biotransformation. this present study, pathway scertained selected isolates measuring their capacity degrade morpholine. Based on analysis culture filtrates, it determined novel isolate genus Halobacillus blutaparonensis which utilizes diglycolic acid route from induce one two branches presence while other branch inhibited. Whatever with regard exhibited bacteria are, ammonia final end product might biochemically utilized isolate.